Detection Reagent A and Detection Reagent B will need to be mixed before processing the samples. Reagent A has a small amount of dark yellow liquid that is slightly viscous and must be pipetted and not poured directly into the Detection Reagent B vial.
- Make sure Detection Reagent A and Detection Reagent B are at room temperature before proceeding (**Do not add to the incubator to warm the reagents**)
- To ensure the reagents are completely transferred, add 1ml of Detection Reagent B (larger tube) into the Detection Reagent A vial using a micropipette.
- Using the same pipette tip, transfer the entire contents of the Detection A vial into the Detection B vial and invert gently several times to mix.
- Transfer 300uL of the Detection Reagent's solution mixture into each of the empty sample/control microtubes numbered in Row 3 of the microtube rack.
The microtubes are now ready for the addition of sample "top liquid" after they have been clarified in the centrifuge.