Are dead Listeria cells or DNA picked up by the DETECT/L assay?

The DETECT/L assay is a phage-based test, which by nature only detects the presence of live/viable Listeria cells.  Unlike PCR-based tests for Listeria, DNA/dead cells are not detected by DETECT/L assay. 

The phage infects live cells and essentially reprograms the cells to produce a specific enzyme, luciferase.  This enzyme, when combined with our Detection Reagents (substrate combined with luciferase), produce light units (RLU - Relative Light Units).  The presence of light is detected with the DETECT/L Reader.  When compared to a negative control the Sample6 software, qualifies the environmental samples as positive or negative for Listeria species based on the amount of light produced by each sample.



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