The DETECT/L assay is a phage-based test, which by nature only detects the presence of live/viable Listeria cells. Unlike PCR-based tests for Listeria, DNA/dead cells are not detected by DETECT/L assay.
The phage infects live cells and essentially reprograms the cells to produce a specific enzyme, luciferase. This enzyme, when combined with our Detection Reagents (substrate combined with luciferase), produce light units (RLU - Relative Light Units). The presence of light is detected with the DETECT/L Reader. When compared to a negative control the Sample6 software, qualifies the environmental samples as positive or negative for Listeria species based on the amount of light produced by each sample.