"DETECT check" is a step of the DETECT/L assay triggered by a "presumed positive" reading - when the sample light signal in RLU is above a validated threshold. This threshold is partially determined by the negative control, specific to each run of the DETECT/L assay.
Running a DETECT check:
- When the DETECT check prompt is showing, open the DETECT Reader door and add the appropriate amount of DETECT check solution with a micropipette.
- Invert the tube 3X to mix the contents of the tube
- Touch the "DETECT check" button on the iPad (left column) and close the door to the DETECT Reader. The sample will be read 10 times and a final "presumed positive" or "negative" read will be assigned.